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However, these compounds were not identified by LC-HRMS/MS, perhaps due to chemical conjugation (such as thiol-DOPA conjugates), degradation, or association with proteins

By contrast, SLC7A11 knockdown increased ECAR and decreased OCR (Supplemental Figure 3A, 3B)

Habib et al

Chromatographic analysis of the GSH-OPA abduct was performed using reverse-phase chromatography on Agilent 1200 HPLC (Agilent Technologies, Stockport, UK) with G1311a Quaternary pump, G1367a Autosampler (5 L injection), G1316a Column Compartment (37 C) G1321B Fluorescent light detector, fitted with Phenomenex Gemini C18 100X2 mm and an additional guard column (Phenomenex, Macclesfield, UK) and a stepwise solvent gradient of 25 mM NaH 2 PO 4 (pH 6.0) and acetonitrile as the mobile phase

Kim K, Choi H, Kim H, Lee T