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How I investigate acquired megaloblastic anemia

8.2.2 Mechanistic and transgenerational research Critical knowledge gaps require focused investigation to establish causality and improve risk prediction

Thirty-five-week-old MC4R mice were randomly divided into four groups with no blinding at one day before drug treatment, vehicle (saline, s.c.), semaglutide (0.5 mg/kg, s.c.), tirzepatide (0.05 mg/kg, s.c.), and retatrutide (0.05 mg/kg, s.c.) based on amounts of food intake from day 25 before drug treatment, body weight at day 2 before treatment, and fat mass at one day before treatment

Mice were given 15 mg/kg in 20 L saline daily between 7 AM and 10 AM over an 8-week period