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Numerous studies have reported that NNMT has an inhibitory effect on the aerobic metabolism of sugars and fats, and NNMT Knockdown can increase oxygen consumption, promote sugar consumption and reduce fat synthesis, but the effects of NNMT on anaerobic energy metabolism have not been reported

Pinch The Skin: Create a firm grasp of the fatty tissue by pinching the skin using your non-dominant hand

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The above procedure was repeated with inhibitor concentrations spanning 0.3 60 M to determine the effective concentration (EC 50 ) required to inhibit 50% NNMT activity in cultured adipocytes