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We were inspired by Glen "BUB" Dohertya Navy SEAL, an adventurer, and a man who lived life to the fullest

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DNA was extracted from the tissue by incubating the ear sample in 1 solution A (Solution A (10): 250 mM NaOH, 2 mM EDTA, in water) at 95 C for 30 min, followed by neutralizing the reaction with 1 solution B (Solution B (10): 400 mM Tris-HCl, in water)

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Expression and distribution of injected viral vectors Immunohistochemistry was used to visualize the distribution of the lentiviral EGFP 3 weeks after injection of the shRNA vector in the striatum of the mice