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Description
Disulfide linkers rely on the intracellular reductive environment to trigger payload release
In particular, we demonstrated that decrement in GSH level, induced pharmacologically by treatment with buthionine sulfoximine (BSO) or metabolically by fasting, resulted in increased NO bioavailability at the muscle level, a condition that could be detrimental [44]
Protein concentration determination Protein concentrations were determined using Quick Start Bradford Protein Assay (Bio-Rad, #5000201) or BCA Protein Assay Kit (Thermo Scientific, #23225) following the standard protocol provided by the respective manufacturers
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JJ Dugoua is a naturopathic doctor in Toronto and has a PhD in Pharmaceutical Sciences
