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Western Blotting Proteins were extracted from chondrocyte (in vitro) and cartilage (in vivo) with cell lysis buffer (Pro-Prep, Intron Biotechnology, Korea) and protein concentration was measured using a BCA kit (Thermo Scientific, USA)

These results suggest that the glutathione system is impaired in its ability to effectively scavenge ROS in the diabetic retina

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f-j , HeLa cells were deprived of methionine for 12 h, treated with Erastin, RSL3, and Fer-1

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