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This GSH conjugation has been shown to occur in mammals, birds, reptiles, amphibians, fish, insects, and other invertebrates [26], and it is the first step of mercapturic acid formation that is one of the metabolic pathways for detoxication of xenobiotics

GenBank AA305647 EST176642 Colon carcinoma (Caco-2) cell line II Homo sapiens cDNA 5' end similar to ribosomal phosphoprotein P0, acidic, mRNA sequence

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The RT-qPCR reaction volume was 20 L, which included 10 L 2 SYBR Premix Ex Taq (Takara Bio, Inc., Kusatsu, Japan), 1 L (10 pmol/L) forward primer, 1 L (10 pmol/L) reverse primer, 2 L template DNA and 6 L sterile water, primer sequence as shown in Table 1

doi: 10.3389/fcell.2022.976953 Received 23 June 2022 Accepted 18 July 2022 Published 10 August 2022 Volume 10 - 2022 Edited by Xinjian Zhu, Southeast University, China Reviewed by Nyzil Massey, Iowa State University, United States Manikandan Samidurai, Iowa State University, United States Updates Copyright 2022 Fabisiak and Patel