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GSH was estimated from the supernatant by 5,5-dithiobis-(2-nitrobenzoic acid) (DTNB) method

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GHK-Cu plays an important role in tissue regeneration, wound healing, and skin remodeling through multiple mechanisms: it promotes the recruitment and activation of repair cells, enhances the synthesis of extracellular matrix components (including collagen, elastin, and glycosaminoglycans), modulates extracellular matrix turnover by increasing both metalloproteinases and their inhibitors, and exhibits powerful anti-inflammatory and antioxidant effects