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Vitamin B12 is often recommended for individuals with documented deficiency or symptoms associated with low levels

Nevertheless, supplementation should ideally be guided by clinical assessment and, where appropriate, blood testing to confirm deficiency before commencing long-term high-dose therapy

Cell death assays using chromium-51 release assay, PI staining, and fluorescent imaging For 51 Cr release assay cells were treated with 51 Cr and incubated for 1 h, after which the cells were washed twice using DMEM media in order to wash the excess 51 Cr

When VB12 deficiency is diagnosed, treatment options include a standardised replacement regimen or close monitoring of VB12 levels and replacement as required

All animal experiments were approved by and conducted in compliance with Institutional Animal Care and Use Committee (IACUC) of the University of Colorado Anschutz Medical Campus