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Briefly, each primary antibody was incubated for 12 h in a humidified chamber at 37 C, followed by detection using the HRP-conjugated secondary antibody and TSA-dendron-fluorophores (Histova, NEON 7-color IHC Kit for cryosection, 1:100), after which the primary and secondary antibodies were thoroughly eluted in stripping solution (Histova, Abcracker) for 60 min at 37 C

Anti-inflammatory effects of plumbagin are mediated by inhibition of NF-B activation in lymphocytes

After stimulation cells were lysed

It explicitly rejects biologics-level evidentiary burdens for every peptide and instead proposes a three-tier system: Tier 1 for endogenous or near-endogenous peptides with cGMP manufacturing, identity/purity/potency testing, basic toxicology, and mandatory pharmacovigilance

Outcome: studies reported at least one of the following outcomes: serum hemoglobin, hematocrit, packed cell volume (PCV), RBC half-life, reticulocyte, blood GSH, and serum VitE levels