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Pregnane X receptor (PXR), constitutive androstane receptor (CAR), and benzoate X receptor (BXR) define three pharmacologically distinct classes of nuclear receptors

Moreover, the levels of GPX4 and FTH1 protein measured by western blotting were upregulated in the xenograft mouse model residual tumors after 5-FU treatment ( Figures 5AC , and Supplementary Figure S4A )

After 1 h incubation (37, 5% CO 2 ), cells were washed twice with warm PBS followed by the addition of 50 l 2 M KOH to solubilize cell membranes and then 50 l of DMSO to dissolve blue formazan with gentle shaking for 10 min at room temperature

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