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Mechanistic evidence relies heavily on animal studies (e.g., mouse neurotransmitter experiments) with insufficient large-scale human randomized controlled trials (RCTs) to confirm causality

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Additionally, we employed two phylogenetic inference techniques, neighbor-joining (N-J) and maximum likelihood (ML), to create phylogenetic trees to validate the topologies because the N-J tree has greater bootstrap values than the other phylogenetic tree, we elected it for our future investigation (Figure 1)