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Further, the amino acid content was analyzed by GC-MS using a Hewlett-Packard 6890N/5975 instrument (Agilent Technologies, Torrance, CA, USA) and a ZB-AAA (10 m 0.25 mm) amino acid analysis column with constant carrier gas flow and an oven temperature program as previously described (Pavlk et al., 2012)

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This allows you to conjugate the antibody to the beads directly, through covalent binding