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The pathophysiology of nociplastic pain is now understood to exhibit significant mechanistic heterogeneity, leading to a clinically and neurobiologically meaningful distinction between two primary subtypes, that is, bottomup and topdown nociplastic pain [56]

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Glutathione is an extremely important antioxidant that can help protect against free radicals
FSP1 is one of the key regulatory molecules involved in ferroptosis, which regulated by upstream factors, including transcription factors and noncoding RNAs (ncRNAs), and is subject to epigenetic modifications, all of which influence the progression of ferroptosis-related diseases [30, 54]

Total protein concentration was measured using a BCA assay kit (TIANGEN)
