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Hellweg R, Raivich G, Hartung HD, Hock C, Kreutzberg GW

The tissue samples were lysed in 1 x lysis buffer (20 mM Tris-HCL [pH 7.5], 150 mM NaCl, 1 mM Na2EDTA, 1 mM EGTA, 1% Triton X-100, 2.5 mM sodium pyrophosphate, 1 mM b-glycerophosphate, 1 mM Na3VO4, 1 mg/mL leupeptin), supplemented with protease inhibitor 1 mM PMSF

Moringa oleifera: A Tree of Life as a Promising Medicinal Plant for Neurodegenerative Diseases

Moreover, aging was associated with the decline of antioxidant response, as some of the antioxidant genes induced by oxidative stress declined with age, such as GSH levels [108]