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Indeed, autoreactive cells and low-titer autoantibodies are commonly detected in healthy individuals and more frequently in siblings of autoimmune patients ( Several tolerance mechanisms exist in the periphery, including clonal deletion, anergy, immune deviation, ignorance, and active suppression by regulatory T cells (Tregs) (49)

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The primary antibody (EM1901-56) at 1/2,000 dilution was used in 5% NFDM/TBST at 4 overnight

Gekka [141] et al

PMID: 31022917 Cancer Res Commun Inhibition of NEK2 Promotes Chemosensitivity and Reduces KSHV-positive Primary Effusion Lymphoma Burden