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Weekly injections over four weeks help to restore and replenish levels effectively, before moving onto single maintenance treatments to keep levels topped up

At the end of each experiment, rats were anesthetized with isoflurane gas, and euthanized with carbon dioxide

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While optimal dosages can vary, clinical settings typically use NAD+ injection doses ranging from 100 mg to 1,000 mg per day

At the same time, it was speculated that the stem cells may down-regulate the expression of IL-6, IL-8, and TNF- through paracrine mechanisms, thus delaying the progression of IDD [35]