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Results The b ar gene (PAT protein, herbicide resistant) was cloned in pGEX-4T-1 and expressed by E

doi: 10.1016/j.lwt.2020.109494 7

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The Level of Intracellular Low-Molecular-Weight Thiols in gmhA Mutants Against the Background of Genetic Modification of Cysteine and Glutathione Metabolism Restriction of cysteine metabolism due to cysB and cysE gene deletions, as well as inhibition of glutathione synthesis ( gshA and gshB deletions) lead to a significant decrease in the level of low-molecular-weight thiols (by 65, 45, and 72% compared to the control, respectively) (Fig

Because the uncatalysed dismutation reaction for superoxide requires two superoxide molecules to react with each other, the dismutation rate is second-order with respect to initial superoxide concentration