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In addition, the immunopositive stained area percentages for MYD88, TLR4, and NF-B were evaluated using the same software applying the color deconvolution plugin and H-DAB vector ( 2.10 Immunoblotting A tissue homogenate was prepared from the testicular tissue, and the protein content was ascertained using the Bradford Protein Assay kit (Bio Basics Inc., Ontario, Canada, Cat# SK3041)
2003 Sep 19;278(38):36887-96
