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Neutral Red uptake assay (above): NHEKs were plated into 48-well culture plates (18,000 cells per well) and allowed to recover for two days in a serum-free medium

After incubation, beads were pelleted by centrifugation and washed 35 times with 0.2% NP-40 buffer (25 mM Tris-HCl, 500 mM NaCl, 10% glycerol, 0.2% NP-40, pH 7.4)

Besides, in the ALD model, L

Zhiyu Yang, Dr
