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doi:10.1186/1472-6815-2-3

By creating various allo-tRNA variants, incorporating the D loop portion from As tRNA Sec , and optimizing SelA and allo-tRNA Sec expression levels using various promoters, a successful system for efficient multiple-site Sec incorporation was established (Figure 10)

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Challenges and pitfalls 6.1 Quench by acidification A key issue in the study of thiol/disulfide equilibria remains the adequacy of the methods used for quenching

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