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Xiong et al., 2024)

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Finally, the sections were counterstained with hematoxylin for 20 seconds and dehydrated

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To determine the amount of NFT required after its initial addition and a washout phase to maintain elevated glutathione levels, cells were pretreated with 20 M NFT for 48 h, maintained in NFT-free medium for 24 h, and subsequently treated with varying concentrations of NFT over 24 h