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Simple visual inspection of the colour gradient gave semi-quantitative data, but the colour coordinates could be employed to obtain quantitative data

Simultaneously, western blot was used to detect the crucial proteins related to the cell cycle

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Unopened vials should be kept at room temperature, as freeze thawing, could result in the stability being damaged or reconstitution failure

Detailed analyses of these approaches emphasise the transformative but not yet realised potential of ML to improve outcomes for individuals with ASD