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To limit glial scar formation, a cocktail of three mitotic inhibitors was added to the medium for the first 3 days of culturing: uridine, 5-fluorodeoxyuridine and cytosine--arabinofuranoside [4.4 mM each, Sigma, #U3003, #F0503 and #C1768]

Second, we again quantified the overlap between generated molecules and rule-based enzymatic transformations predicted by BioTransformer within each sampling frequency bin