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Serum was separated by centrifugation for 30 minutes at 3000 rpm and 4C after collecting 1 ml of blood by cardiac puncture

Gastrointestinal issues are frequently the adverse effect of metformin medication that is most commonly observed, affecting 2-63% of T2DM patients, including diarrhea, nausea, vomiting, pain in the abdomen, and flatulence [47]

For HPLCMS analyses, data collection was carried out with OpenLAB CDS ChemStation Edition (C.01.07 SR4), while for data analysis the OpenLAB CDS (2.4 Update_06) was used

It is an animal study with a relatively small sample size and little clinically correlated data

But as for high doses of vitamin E, obtained from the Internet or local stores, it is a different story