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Consequently, FUNDC1 phosphorylation at Tyr18 and Ser13 undergoes dephosphorylation, resulting in a conformational change and elevation of the FUNDC1 affinity for LC3 or GABARAP

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The reaction was allowed to proceed for 10 to 20 min and was stopped by the addition of 450 L of Purpald solution (22.8 mM Purpald in 2 N potassium hydroxide)

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