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a combination of 1mM IBMX, 1 M dexamethasone, and 10 g/ml of insulin in media were added to fully confluent 3T3-L1 fibroblasts for three days (days 03) to initiate differentiation

Knockdown of NNMT elicits heightened energy expenditure in adipose and hepatic tissues, mitigates lipid accumulation, and enhances insulin sensitivity