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Overexpressed proteins were purified on a Ni-NTA column (Cytiva) with an elution buffer containing 50 mM NaH 2 PO 4 , 300 mM NaCl, and 300 mM imidazole
During the 8-week intervention, patients were assigned to either a high-carbohydrate diet group (7 patients) or a KD group (also 7 patients)
10.3344/kjp.2022.35.3.291 21 FreireM
According to reports, curcumin is a P450 inhibitor that inhibits CYP2C9, CYP1A2, CYP2D6, CYP2B6 and CYP3A4, with particularly low IC50 values for CYP2C9 (Appiah-Opong et al., 2007)
