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Each reaction was performed in triplicate and run on the Standard Real-Time PCR program on a 7900 Taqman real-time PCR system (Applied Biosystems, Warrington, UK) using pre-defined thermal cycling conditions (50 C for 2 min, 94.5 C for 10 min, 40 cycles of (97 C for 30 s and 59 C for 1 min))

Ongoing refill reminders are available for continued wellness support

[9] If you experience side effects from ED medications, report them through the MHRA Yellow Card scheme (yellowcard.mhra.gov.uk)

J., Dewhirst, F