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The culture medium was refreshed 6 h post-transfection

Primers for SEMA6A were: ACCTGTATTGCCTCCAGAGACC and CCAGACCATCTGTATTGCCACG

The ability of eNOS produced NO to prevent platelet adhesion and aggregation reduces the propensity for the development of abnormal fibrin clots (thrombi) and, therefore, will contribute to a reduced potential for the development of atherosclerosis

Beyond GI benefits, supports healing throughout the body including tendons, ligaments, muscles, and joints through systemic distribution and growth factor modulation pathways

Ethics declarations Human and Animal Rights All reported studies/experiments with human or animal subjects performed by the authors have been previously published and complied with all applicable ethical standards (including the Helsinki declaration and its amendments, institutional/national research committee standards, and international/national/institutional guidelines)