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[DOI] [PMC free article] [PubMed] [Google Scholar] [100].Holmstrom KM, Finkel T, Cellular mechanisms and physiological consequences of redox-dependent signalling, Nat

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Sixteen hours after transfection, biotinylation was triggered by adding biotin to a final concentration of 50 M (1 mM biotin stock in PBS and stored for up to 6 months)