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Grimacing behaviors in rodents (mice and rats) have been monitored either via manual video scoring or automated via imaging software that is able to capture and score stereotypical headache-like responses, including orbital tightening, whisker changes, ear changes as well as cheek flattening after migraine-inducing agent administration (Langford et al., 2010

Briefly, 10 6 BEAS-2B cells or HBEC were recovered using EDTA-free trypsin, counted and incubated in serum-free DMEM with 0.2 M Calcein-AM at 37 C for 7 min

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The pharmacological treatment options for pediatric migraine: an evidence-based appraisal