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Cells were then harvested by trypsinization, fixed with 4% paraformaldehyde at room temperature (RT) for 15 min, and permeabilized with 0.3% Triton X-100 in PBS at RT for 10 min

In vivo , this mutant tRNA sec decreases the expression of selenoproteins with the greatest suppression found in the expression of GPx-1 (261)

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[DOI] [PMC free article] [PubMed] [Google Scholar] 118.Chang C.H., Tsai W.C., Lin M.S., Hsu Y.H., Pang J.H
