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Description
The beads were then washed and boiled with 1 SDS loading buffer for SDSPAGE analysis, followed by Coomassie blue staining for liquid chromatography (LC)MS analysis, or the proteins were transferred onto nitrocellulose membrane and incubated with antibodies
3 mg/ml in DMSO) and analyzed using LC-MS as described 18
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Sildenafil does not improve cardiomyopathy in Duchenne/Becker muscular dystrophy
doi: 10.1038/nrn3820
