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Description
Samples were then reduced with 10 mM TCEP for 30 min at room temperature, followed by alkylation with 15 mM iodoacetamide for 45 min at room temperature in the dark

Any filler in the wrong hands may cause complications including damage to the skin and/or blindness

RNA Isolation, Reverse Transcription, and Quantitative Real-Time PCR Total RNA was isolated from whole blood samples using Trizol LS reagent (ThermoFisher Scientific, Rockford, IL) according to manufacturer's recommendations

However, stacking increases complexity and potential risk especially if done without medical guidance
doi:10.5935/1518-0557.20180046
