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Aggregates (56/well) were transferred to collagen-coated transwell inserts (Corning, #3491), treated with 5 M CHIR99021 for 1 h, then cultured in differentiation medium containing 100 ng/mL FGF9 and 100 ng/mL BMP7 (PeproTech) for 1014 days with daily medium changes, avoiding overflow onto the apical surface

doi: 10.1002/fsn3.70587, 189

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