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RT-PCR Total cell RNA extraction was performed using TRIzol (Invitrogen) and reverse transcribed according to the manufacturers protocol (Takara)

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Taking into account that TNF and ROS may induce NF- B-dependent gene transcription, this potentially results in an amplification loop of TNF and ROS signaling [269]

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Immunology (2018) 154(2):17885

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Partial restoration of mutant enzyme homeostasis in three distinct lysosomal storage disease cell lines by altering calcium homeostasis

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