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Total protein concentration was determined by BCA assay kit (ThermoFisher, no
CTR1: copper transporter 1

It is recommended to be used as part of a healthy diet including regular consumption of fruits and vegetables and does not substitute a normal diet
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Furthermore, an additional transcript was observed using the gadR-Fw/gadC-Rv primer set (1,903 bp), encompassing the gadR and gadC genes, indicating the bicistronic nature of these genes (Figure 1)

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