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2015;10(7):e0131177

Methyl-B12 is closely allied with the folic acid biochemical pathway

Microglia control the glycinergic but not the GABAergic synapses via prostaglandin E2 in the spinal cord

Li L, Ameri AH, Wang S, Jansson KH, Casey OM, Yang Q, et al

For measurement of metabolite levels in cells, hepatocytes were harvested by treating with 0.25 % trypsin (Gibco #25200), washing with ice-cold PBS to remove trypsin, suspending released hepatocytes in ice-cold PBS, and centrifuging the cell suspension at 1600 g for 10 min at 4 C to obtain the pelleted cells, which were immediately frozen and stored at 80 C for later analysis