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These repeated assessments verified that hyperglycemia was maintained in all diabetic groups throughout the experimental period, minimizing inter-group variability and confirming the validity of the diabetic model prior to downstream functional analyses

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Therefore, metabolic tests based on biomarkers that identify autism-associated metabolic subpopulations will require sensitivities that detect low prevalence metabolic phenotypes, have high specificities to distinguish the phenotype and, ideally, provide new or support existing biological insights

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