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For example, this study by Funk et al

And neither should its preparation

Boosting NAD level suppresses inflammatory activation of PBMCs in heart failure

Nakamura T, Takagaki K, Shibata S, Tanaka K, Higuchi T, Endo M

Retina immunofluorescence Retinas were dissected, cleaned and dehydrated in 100% ethanol for 30 min followed by permeabilization with 1% Triton X-100 for 30 min at RT