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After washing once with PBS to remove unbound CFSE, SFN was added for 30 min and the cells were seeded on 96-well microplates coated with anti-CD3/CD28 antibodies for co-stimulation as described above

Resolutions were estimated using the gold standard criterion (FSC = 0.143), and the local resolution was calculated in cryoSPARC

Our categorization is well in agreement with previous rating standards (Metson and Brooks, Figure 4C)
This means you get better results while having to use less.* Sustamine? enhances performance and recovery in three primary ways

Carmichael RE, Wilkinson KA, Craig TJ
