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New perspectives on nanomaterial aquatic ecotoxicity: production impacts exceed direct exposure impacts for carbon nanotoubes

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Briefly, the kanamycin resistance cassette of strain UC1098. ptsG ( ptsG:: FRT kan FRT) (see below) was exchanged by a PCR product harbouring the ptsGlinkermNG fusion gene flanked by upstream/downstream chromosomal regions of ptsG using growth on M9 minimal medium with glucose (0.4% (w/v)) for selection with ptsGmNG expressing colonies being significantly larger compared to those of precursor strain ptsG

For protein separation, SDS (a detergent molecule) helps denature (unfold) the protein and gives it a consistent size-to-charge ratio

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