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The harvested cell pellet was resuspended in 50 mM Tris-HCl pH 8.0, 300 mM NaCl, 10 mM imidazole and 0.05% 2-mercaptoethanol (lysis buffer) and lysed by sonication

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doi: 10.3390/pathophysiology31020019, PMID: [DOI] [PMC free article] [PubMed] [Google Scholar] 6

Clinical trials have also reported that onion (quercetin-rich source) intake for 24 weeks reduced cognitive decline, possibly by reducing depressive symptoms and improving emotional state, compared to placebo food group, in Japanese individuals of age-group 6079 years [97]