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Reduction and alkylation was performed by adding Tris(2-carboxyethyl)phosphine (TCEP) and 2-Chloracetamide (CAA) at the final concentrations of 10 mM and 40 mM, respectively, and incubating them for 5 min at 45 C

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[DOI] [PubMed] [Google Scholar] Billeci T

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