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After centrifugation at 65,000 g for 30 min, the supernatant was collected and incubated with 10 ml Ni-NTA resin (Cytiva) in the presence of 8 mM imidazole to prevent the nonspecific binding at 4 C overnight

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Zinc deficiency drives Th17 polarization and promotes loss of Treg cell function

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3.6 Metabolomics and proteomics biomarkers Small molecule metabolite remains the center in the study of metabolomics, and modifications in metabolite levels may be the final result of controlling protein expression