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Cells were analyzed in duplicates using flow cytometry (FACSCantoII, BD Biosciences, San Jose, CA, USA)

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"Food intake reductions and increases in energetic responses by hindbrain leptin and melanotan II are enhanced in mice with POMC-specific PTP1B deficiency." American Journal of Physiology-Regulatory, Integrative and Comparative Physiology

Cells were added to high precision #1.5 coverslips (Corning, Millipore Sigma), placed on a 2% (w/v) agarose pad in LB and imaged at 37 C on a Nikon Ti inverted microscope equipped with a 561-laser line, a Plan Apo 100x/1.4 Oil Ph3 DM objective and a Andor Zyla 4.2 Plus sCMOS camera