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Thymocyte isolation and primary culture was performed as follows: briefly, the thymus from Balb/c mouse was rolled on a piece of sterile gauze to remove attached fat and connective tissue and the gland was placed into a 60 mm Petri dish containing 5 mL of cold media (HBSS at pH 7.0 containing 5% fetal calf serum at 4C) and gently teased the thymus apart with needles and pipeted up and down several times to carefully break up any cell clumps

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