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Strains carrying an HA-tagged version of Adh2 were generated by similar approaches using pYM-N16 (EG103 pGPD-3HA-ADH2 and EG110 pGPD-3HA-ADH2 ) or pYM-N14 (EG103 ADH2-6HA and EG110 ADH2-6HA ) as templates and primers ADH2_S1 and ADH2_S4 or 5-GGCATACTTGATAATGAAAACTATAAATCGTAAAGACATAAGAGATCCGCTTAATCGATGAATTCGAGCTCG-3 (ADH2_S2) and 5-AGATGGAGAAGGGCCAAATTGCTGGTAGATACGTTGTTGACACTTCTAAACGTACGCTGCAGGTCGAC-3(ADH2_S3), respectively

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