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After 72 h drug exposure cell viability was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT)-vitality assay (EZ4U, Biomedica, Vienna, Austria) following the manufacturers recommendations, as published 55

Remarkably, PP2 altered the GSB-106-elicited Tyr 706/707 and Tyr 515 phosphorylation more profoundly than that induced by BDNF, whereas the decrease of Tyr 816 phosphorylation was less striking in case of GSB-106

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Thats enough to raise red flags on infection risk, dosing uncertainty, and injection site reactions

Buckley L, Guyatt G, Fink HA, et al